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Speaker 1: What's up everyone, and welcome back to the program. In

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this episode, we're taking a dive right back into those

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core documents and we're going to take a look at

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the notice of filing Affidavid of Leo Larkin in support

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of defendant's third motion to compel. So what this is

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is the defense Brian Colberger's team. This is one of

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their experts who is putting in an Affidavid of support

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for the defense to get a look at the genealogical

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and DNA evidence that's been collected. So let's get into

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this document and let's see what Brian Coolberger and his

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team are bringing to the table. Case number c R

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twenty nine DASH twenty two DASH twenty eight oh five

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Notice of filing Affidavid of LEYA. Larkin in support of

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defendants third motion to compel The State of Idaho Plaintiff first,

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Brian C. Coberger. The defendant comes now Brian C. Koberg

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buy and threw his attorney of record and C. Taylor,

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public defender, and hereby files the attached affid David of LEA.

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Larkin in support of the Defendant's third motion to compel.

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This was dated on August ninth of twenty twenty three,

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and it was signed by Ann Taylor. The next document

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is the Affid David of Leah Larkin Case number c

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R twenty nine DASH twenty two DASH twenty eight oh

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five the State of Idaho Plaintiff verse Brian C. Coberger

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the defendant, State of California, County of Alameda. One. I

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am an adult over the age of eighteen, and I

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have personal knowledge of the facts set forth in the

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Affid David two. I have been engaged as an expert

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in the above entitled matter since July twenty seventh, twenty

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twenty three three. I am a genetic genealogist, having practiced

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genetic genealogy since twenty fourteen. My educational background is a

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bachelor's degree in biology and a PhD in botany. My

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curriculum VITA is attached. As a genetic genealogist. I have

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a clear understanding of how genetic genealogy works. I have

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developed tools that are in widespread use by genealogy practitioners.

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Number four. Genetic genealogy is best described as the use

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of DNA data to evaluate biological relationships. There are three

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categories of genealogical DNA testing. Why chromosome DNA direct paternal

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line only, mitochondrial DNA direct maternal line only, and autosomal

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DNA all branches of a pedigree. A genetic genealogist integrates

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information from these DNA tests with genealogical documentation to reveal

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or corroborate relationships. Genetic genealogy primarily uses autosomal DNA tests

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that were originally designed for biomedical research. Since twenty three

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and Meters brought their health test to the consumer market

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in late two thousand and seven, family Tree DNA, Ancestry DNA,

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my Heritage, and a handful of smaller companies have launched

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their own genetic genealogy products. These companies all provide genealogy

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tools to their customers on their websites and also produce

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a raw DNA data file that the customer can download.

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The raw data file contains the genetic profile for that customer.

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This genetic profile is not the same as an STR

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profile that is commonly uploaded to codis in a forensic

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DNA analysis number six. Most of the human genome is

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identical across all people. Only the DNA positions at very

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between individuals are useful for genealogy. These variable positions are

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called single nucleotide polymorphisms SNP pronounced SNIP. The direct to

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consumer genealogy companies use a type of test called a

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micro array that samples roughly six hundred thousand of these

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DNA SNPs. Unlike the str markers used for matching in CODIS,

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SNPs can convey information about medical and physical traits, as

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well as biological relationships to close and distant cousins number seven.

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A micro array SNP profile is sometimes called a kit.

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Once the lab test is complete, the genealogy company compares

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the new kit to those already in its database, looking

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for long stretches of SNPs that are compatible. The company

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then presents these matches in a list sorted by the

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amount of shared DNA measured in Cena Morgans abbreviated CM

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number eight. A standard DNA micro array requires approximately two

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hundred enngs of quality DNA. That quality and quantity is

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often not available from crime scenes or degraded human remains. Instead,

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specialized laboratories can generate an SNP profile through a more

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complex process. First, they attempt to sequence the entire genome

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using Next generation sequencing NGS technology. This technology works a

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bit like skimming while reading a document. On the first pass,

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NGS technology will not capture all of the data, and

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it may make mistakes. NGS involves repeated passes called coverage

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to get more accurate results. For human genome sequences thirty

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times to fifty times, the coverage is recommended Number nine.

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Once the NNGS file is obtained, it must be manipulated

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through bioinformatics to make it compatible with the genealogy databases.

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The extra data must be stripped out to just the

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SNPs that the genealogy companies test, and missing or ambiguous

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data must be inferred using statistical methods. The kit is

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then uploaded to a genealogy database and evaluated. A poor

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quality kit might have too few matches, or it might

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have phantom matches that are not real measures of relationship.

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Often bioinformatics step must be repeated. This trial and error

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process can take several tries to produce a kit that

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works well in a genealogy database. Number ten. Only ged Match,

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Family Tree DNA and some smaller entities allow crime scene

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and human RENS main samples to be uploaded. The terms

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of service of Ancestry DNA twenty three in Meters and

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My Heritage prohibit forensic investigative genetic genealogy in their databases. However,

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in the absence of an effective oversight, forensic genetic genealogists

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are on an honor system to obey the terms of

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service and the Department of Justice Interim Policy on forensic

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genetic genealogy. Number eleven. Each of the genealogy databases compares

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the users DNA data to all of the other data

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files in their database, then presents a list of DNA

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matches who share meaningful amounts of DNA. The match list

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includes the match name or elias, how much DNA they

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share in Senta morgans, and occasionally a link to a

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family tree that the match has voluntarily provided. Number twelve.

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The genetic genealogy databases are siloed from one another. If

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you test at ancestry DNA and your sibling tests at

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twenty three in meter, you will not be matched to

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one another because you are not in the same database.

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Ged match is a third party site that functions as

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a genetic genealogy commons. A user can upload their raw

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DNA data file from their testing company and find matches

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to people who upload to ged match. From different companies

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like ged match. Family Tree, DNA and my heritage also

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allow uploads of raw data files, and ancestry DNA and

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twenty three in me do not thirteen. Broadly speaking, the

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more DNA two people share, the more closely they are related.

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The correlation is not perfect, though, any given sent the

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Morgan amount can represent more than one possible relationship. The

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testing companies suggest probable relationships, but experience genetic genealogy typically

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use the online Shared CM tool shown at right for

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a hypothetical match of two hundred cms to see mathematical

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probabilities of different relationships for a given center Morgan amount.

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Note that there are nearly twenty possible relationships listed for

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a match of two hundred CM, which is considered a

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fairly close match. Number fourteen adoptees have used genetic genealogy

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DNA tests to identify their biological families for roughly fifteen

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years now. The same methods used for people with unknown

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parentage can be used to identify forensic samples from a

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crime scene or unidentified human remains. Unlike standard forensic SDR tests,

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which are used to identify individuals, genetic genealogy testing identifies

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possible relatives number fifteen. First, the genealogist attempts build family

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trees from the DNA matches of the person of interest,

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starting with the closest matches. Relatively few users at ged

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match and family tree DNA post their pedigrees there, so

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the genealogists must build trees for them. We attempt to

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identify the match using their screen name and email address.

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Then we search public records, social media, obituaries, news articles,

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and genealogy websites to build out their tree through their parents, grandparents,

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and so on. Most genetic genealogists build these so called

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research trees at ancestry dot com number sixteen. The goal

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of building trees is to figure out how two or

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more matches are related to one another. For example, if

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the person of interest matches two people who share a

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great grandparent couple, making them second cousins to one another,

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the genealogists now knows which branch of their tree is

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relevant to the search. The person of interest could be

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descended from that couple, or the connection could be one

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or more generations further back through either the husband or

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the wife in that couple. Number seventeen. Once several of

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the DNA matches have been connected to one another, the

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genealogists must figure out how the person of interest fits

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into the tree of those matches. We use age, sex,

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geographic location, and other circumstantial evidence alongside the DNA based

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relationship predictions. A statistical tool called what are the Odds

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can analyze multiple DNA matches together to evaluate where the

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person of interest best fits into the tree based on

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the amounts of shared DNA number eighteen. What are the

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Odds is not intended to give a definitive answer. Rather,

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it points the genealogists toward the most likely branches in

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the tree for further research. The tool is also not

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appropriate for all circumstances. For example, it is not intended

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for populations that married within themselves, for double cousins, or

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when most of the matches are below forty CM number nineteen.

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The genealogist needs to do this for both the maternal

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and paternal sides of the person of interest pedigree. Often

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this involves building dozens of trees and performing several what

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are the odds analysis. We are looking for instances where

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a descendant in one DNA family tree married a descendant

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in another DNA family tree, because those unions point to

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where the person of interest might fit in to the

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family tree. Often, the best we can do with the

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existing DNA matches is to focus on a set of

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cousins as candidates. Then more DNA testing is required Number

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twenty This process can vary in complexity depending on the

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individual starting point. In doing this type of research, the

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genetic genealogist will generate numerous documents to ensure an adequate

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paper trail. This paper trail can include the list of

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DNA matches, the research tree with genealogical documentation, public record

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searches for members of the DNA family, correspondence with DNA matches,

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descending diagrams showing how the DNA matches are related to

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one another, and what are the odds analysis. The documentation

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is necessary and important for the following reasons. DNA matches

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sometimes hide their profiles. An error in the tree can

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mislead the genealogist and the descendant. Diagrams and what are

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the odds analysis are usually updated repeatedly during a search.

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Number twenty one. As a genetic genealogist, I have learned

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about the power and the privacy implications of genetic genealogy.

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The tests themselves were designed to reveal biomedical information and

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can also reveal family secrets about the tester. In many cases,

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private information can be inferred about the DNA relatives of

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the tester as well. For those reasons, leaders in the

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genetic genealogy community develop standards that emphasize consent and privacy.

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Number twenty two. I am aware of the Department of

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Justice interim policy to limit when forensic genetic genealogy can

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be used and which databases are accessible to law enforcement.

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It is possible for the databases to be utilized in

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a way that circumvents these core principles. Notably, I'm aware

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of a case in which the chain of custody failed

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and the wrong SNP profile was sent to the wrong client.

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Investigative genetic genealogists uploading SNP profiles to a forbidden database

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in violation of that company's terms of service, and the

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Department of Justice in our own policy c forensic genetic

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genealogy being used for a case that did not meet

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the Department of Justices threshold d Investigative genetic genealogists using

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security loopholes to see DNA kids who are opted out

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of forensic matching at ged match N family Tree DNA

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E A case in which an SNP profile was performed

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on an innocent woman, a potential Fourth Amendment violation to

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a write to privacy and uploaded to ged match without

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her knowledge or consent. Number twenty three Having access to

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the data and the methods used to identify someone will

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provide answers to a compliance with Department of Justice policies.

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B how a DNA sample was handled from the time

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of collection through its forensic genetic genealogy testing C, the

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process of SNP creation, D the use of the SNP

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profile E. The use or lack thereof of loopholes and

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violations of terms of service to the various genetic genealogy databases. Further,

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you're affient saith not dated this eighth day of August

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twenty twenty three and signed by Leah Larkin. Now after this,

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there's a bunch of pages of her schooling and what

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she has done as a professional. We're not going to

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dive into all of that, but the link, like usual,

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will be in the description box if you'd like to

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go and take a look. All right, folks, that's going

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to do it for this one. All of The information

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that goes with this episode can be found in the

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description box.

